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LCMS

CLAM 2000

The CLAM-2000 fully automated sample preparation module for LCMS is based on the extensive blood coagulation analyzer technology that Shimadzu has cultivated over many years. Simply place blood collection or other tubes in position and the system fully automates everything from pretreating blood or other samples for analysis to LCMS analysis. Consequently, the system is able to minimize human error and variability in sample pretreatment procedures. Therefore, it helps achieve a high-precision workflow for clinical research that is safer, faster, and simpler.

Seamless Integration of Sample Preparation and LC-MS/MS Analysis.

  • Simply place the blood collection tubes to be analyzed, necessary reagents, and specialized pretreatment vials into the CLAM-2000 which then performs all processes automatically.
  • Each sample is processed successively in parallel, to optimize instrument usage and sample throughput.
  • The CLAM-2000 frees up time spent by laboratory staff performing tedious sample preparation steps, thus increasing laboratory efficiency.

Thorough Precision Control of Analytical Results.

  • Easy to access software controlled management of reagents, calibration curves, control samples, and system maintenance ensures system performance and reliability.
  • Easy to interpret charts and figures alert users to decreasing reagent volumes or variable QC sample results.

Reduces Operator Errors and Enhances Laboratory Safety During Procedures.

  • Eliminates manual operations involving biological samples, such as dispensing. Not only does this improve reproducibility of data, but it minimizes user contact with biohazardous material.
  • Potentially infectious waste products are kept isolated inside the system. This allows such waste to be disposed of after the analysis is finished, according to the procedures specified for that laboratory.

Perform All Processes from Collection of Samples in the Blood Collection Tubes to Sample Pretreatment and Analysis Automatically

User-Friendly Experimental Design and Execution

Depending on the samples being analyzed, operations involve two simple steps – placing the blood collection tubes and reagents in the system and then requesting the desired sample analyses in the software. Using a liquid crystal touch panel display, the CLAM-2000 interface was designed to make LCMS sample preparation and analysis accessible to all users, regardless of the level of experience. The CLAM-2000 offers a next-generation workflow for routine LC-MS/MS analysis that helps ensure reliable and rapid operations.

Advanced options for displaying, reporting, and managing data results can be easily accessed through the CLAM-2000 software by linking to LabSolutions.
Note: An optional LCD screen can be installed for monitoring LCMS analysis.

Precise Control of Analytical Results

Improves Accuracy and Reproducibility of Analytical Data

Sample batches are easily created through a barcode reader or by direct entry to facilitate sample analysis. CLAM-2000 software ensures reliable results and continuous operation by tracking solvent levels, calibration curves, QC samples and instrument parameters. These measures help to ensure a routine analysis workflow that continues to provide high-quality results.

The Benefits of CLAM-2000 Automation on LC-MS/MS Workflows

Seamless Automation

The CLAM-2000 not only automates common steps in LC-MS/MS sample preparation protocols, but it delivers the sample to the LC autosampler for injection. Most often, sample preparation involves protein precipitation and derivatization and the CLAM-2000 provides all of the functions required to execute these critical steps.

Reduces Operator Errors

Once the samples and the filtration/collection vials are loaded into the sample carousel, users use a touch panel screen to queue the samples and order the methods. Once loaded, all steps are automatically performed including the LC-MS/MS analysis. Removing the operator from the process in effect removes operator error, thus increasing reproducibility.

Increased Laboratory Efficiency and Safety

Laboratory staff will no longer be tied to a bench, processing samples. Operators will simply need to load samples and reagents, create the batch and press start. Exposure to samples are minimized and all potentially hazardous waste is isolated inside the CLAM until it can be disposed of according to proper laboratory safety procedures.

CLAM-2000-LC-MS/MS Application Examples

Steroids

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a fully automated preparative LCMS analysis system consisting of a Nexera X2 Ultra High Performance Liquid Chromatograph, LCMS-8060 Triple Quadrupole Mass Spectrometer, and CLAM-2000 fully automated sample preparation module for LCMS.

Pretreatment Conditions and Analysis Flow

Quantitative results of drug spiked into serum

CompoundsPolarityRange
(ng/mL)
r2QC samples concentration (ng/mL)Accuracy (%)%RSD (n=3)
LLOQMiddleULOQLLOQMiddleULOQLLOQMiddleULOQ
 Aldosterone+0.03 – 1.140.9970.030.461.1496.5102.0102.86.37.93.6
 Cortisol+1.51 –
320
0.99915.120.8320104.4100.498.61.60.50.7
 DHEAS+12.9 –
2750
0.99912.91792750104.3101.399.63.93.61.9
 Corticosterone+0.29 –
62
0.9980.294.0362101.8101.098.44.60.81.0
 11-Deoxycortisol+0.08 –
18
0.9970.081.1718103.0102.796.15.50.90.2
 Androstenedione+0.08 –
18
0.9990.081.1718116.599.1100.14.61.31.1
 Testosterone+0.03 –
7.2
0.9990.030.477.293.8101.4100.13.71.01.0
 17-OHP+0.12 –
26
0.9980.121.6926103.6103.399.66.21.61.8
 DHEA+0.31 –
65
0.9990.314.226593.198.199.06.53.96.5
 Progesterone+0.12 –
26.5
0.9990.121.7226.5103.3101.099.23.91.11.5
 Aldosterone (neg)0.03 –
1.14
0.9960.030.461.14115.097.397.46.67.58.0
 DHEAS (neg)12.9 –
444
0.99712.920.8444101.7103.099.72.11.27.1
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LCMS-8050

UFscanning

High-Sensitivity & High-Speed Scanning at 30,000 u/sec

Simultaneous Quantitative and Qualitative Analysis

Simultaneous High-Speed Screening of 12 Toxicological Drugs

The LCMS-8050 is capable of simultaneously obtaining both qualitative and quantitative information in a single analysis. Acquisition occurs so rapidly that MS/MS scans and MRM measurements can be performed concurrently while maintaining quantitative accuracy. MS/MS scans are usable and reliable because even at 30,000 u/sec, Shimadzu uses a 0.1 u scan step.

UFscanning

MRM Triggered Product Ion Scanning of a Mixture of 12 Benzodiazepines (1 ng/mL each)

 

UFswitching

High-Sensitivity & High-Speed Positive/Negative Ionization Switching in 5 msec

Just One Minute per Analysis

A Case Study Using High-Speed Positive/Negative Ionization Switching

When performing simultaneous positive/negative ion measurements of multiple components, the proper acquisition of sharp UHPLC peaks depends on rapid polarity switching. The LCMS-8050 minimizes losses due to polarity switching and ensures the collection of sufficient data points for even the narrow peaks obtained with UHPLC, recording accurate peak shapes and allowing excellent reproducibility.

UFswitching

UF-MRM

High-Sensitivity & High-Speed MRM at 555 ch/sec

Detect Target Compounds at Trace-Level Concentrations

Simultaneous Analysis of 29 Pesticides for Water Quality Analysis

The LCMS-8050 is capable of simultaneously acquiring 555 MRM transitions per second while maintaining accuracy and precision. Sufficient data points can be collected for quantitation ions, reference ions, and internal standard ions even in chromatographic regions with unresolved peaks. The high sensitivity of the LCMS-8050 allows for trace-level analysis, such as pesticides in drinking water, without the need for sample pre-concentration. This high sensitivity is maintained even when monitoring numerous MRM channels.

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LCMS-8060

The LCMS-8060 is a ground breaking innovation in mass spectrometry helping to transform LC/MS/MS data quality by developing a new vision of sensitivity and speed.
Shimadzu is the first mass spectrometry company in the world to deliver a triple quadrupole mass spectrometer with the highest sensitivity and ultra-fast technologies to create an innovation that really matters.

 

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LCMS-2020

Ultra high-speed mass spectrometer compatible for the first time with ultra high-speed liquid chromatography

UFLC Quality – Speed is Power.

LCMS-2020

High-speed HPLC analysis to improve lab analysis productivity began in 1982 with the Shimadzu LC-5A/FLC column. In 2006, the Prominence UFLC/XR column system achieved ultra high-speeds while ensuring repeatability during continuous multi-sample analysis, in addition to maintaining durability and other forms of data stability. Since general HPLC analysis is insufficient with respect to qualitative capabilities, risks related to overlooking impurities that can obscure peaks remain. Mass spectrometers are effective at minimizing these risks. To date, however, a commercial MS capable of reliably providing the kind of sharp peaks obtained with ultra high-speed LC has not been available.

Now, an ultra high-speed MS, compatible for the first time with ultra high-speed LC, has been released

The ultra high-speed LC/MS Prominence UFLC+LCMS-2020 improves the speed and reliability of HPLC analysis, offering dramatic improvements in lab productivity.

3 points yielding ultra high-speed, ultra high-sensitivity MS measurements

By incorporating the following innovative features into the LCMS-2020, the sharp peaks of ultra high-speed LC won’t be missed:

  • UFswitching (high-speed positive/negative ionization mode switching)
  • UFscanning (high-speed scan measurements)
  • UFsensitivity (superior sensitivity and repeatability thanks to newly developed ion optics)

The UFLC/MS, which continues the tradition of high-level data reliability from the well-received ultra high-speed LC Prominence UFLC, has now been released.

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LCMS-8030

Ultra Fast Mass Spectrometry -Speed Beyond Comparison-

World Fastest* Tandem Quadrupole LC/MS/MS System

Provides Speed Beyond Comparison for Multi-Analyte Determination

Triple Quadrupole Mass Spectrometry is the method of choice for accurate quantification and confirmation of trace level analytes in complex matrices. From the detection of drugs and metabolites in biological specimens to environmental contaminants and pesticides in food, analysts the world over are challenged with detecting an increasing number of target analytes with greater sensitivity and in more samples than ever before. Shimadzu understands these requirements. That is why we have combined our experience from different fields to build a mass spectrometer that can keep pace with the chromatographic resolving power of our world-leading UHPLC systems without any speed limitation. The LCMS-8030 couples the power of a Triple Quadrupole Mass Spectrometer with unparalleled speed to provide the ideal compliment to both UHPLC and HPLC systems.

With Ultra Fast MRM transitions and Ultra Fast polarity switching, LCMS-8030 is truly a universal detector for UHPLC or HPLC.

* Based on our survey (as of August 2010)

Ultra-High Speed

Introducing the Next Generation Triple Quadrupole Technology

UFsweeper™ Collision Cell

UFsweeper™ Collision Cell

  • Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching of 15msec
  • An Ultra Fast Scan Speed of 15,000 u/sec
  • 500 MRMs per Second, the Most Ever Possible

 

 

 

High Reliability

Even during Ultra Fast Measurements, the LCMS-8030 Provides Reliable and Reproducible Data

  • Robust System Provides Long-term Data Stability
  • UFsweeper™ Technology Dramatically Minimizes Cross Talk
  • Excellent Linearity with Wide Dynamic Range

User-Friendly

The Strength of a Single-vendor Solution Results in Seamless Operation

  • Minimize Instrument Downtime with Easy Maintenance
  • Single-vendor Solution Provides Seamless Operation
  • Quantitation Browser for Effective Multi-analyte Quantitation

Brief explanation of LCMSMS terminology

MRM

MRM stands for Multiple Reaction Monitoring. In MRM, a specific ion (precursor ion) is selected in Q1 (first stage MS) from among various ions ionized by the ionization probe, the ion is destructed (collision-induced dissociation) in the collision cell (Q2, collision chamber), and a specific ion is detected from among the destructed ions (product ions) in Q3 (second stage MS). Multiple channels can be set in one measurement.

Method Packages

In accordance with regulations, the analysis of trace amounts of components containing many contaminants is now required for residual pesticides in foods and veterinary drugs. LC/MS/MS is often used for these analyses. These method packages are pre-registered with the analysis conditions and compound information of target components. Analysis can now be started without performing difficult or time-consuming tasks required in LC/MS/MS analysis, such as evaluating the separation conditions and optimizing the MS parameters for each compound, to allow efficient batch analysis of multiple components.
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LCMS-8040

LCMS-8040 Enhanced Sensitivity

 

LCMS-8040 incorporates newly improved ion optics and collision cell technology to provide higher multiple reaction monitoring (MRM) sensitivity.

These improvements also yield higher sensitivity for scan mode measurements, expanding the potential range of LC/MS/MS applications.

Ultra-High Sensitivity

By incorporating newly improved ion optics and collision cell technology, the LCMS-8040 provides higher multiple reaction monitoring (MRM) sensitivity. A five-fold increase in sensitivity (reserpine, S/N ratio) has been achieved by improving ion focusing and minimizing ion losses between multi-pole lenses. These improvements also yield higher sensitivity for scan mode measurements. This higher sensitivity expands the potential range of LC/MS/MS applications.

Ultra-High Speed

The LCMS-8040 was designed to provide significantly higher sensitivity while maintaining the high speed offered by the LCMS-8030. Ultrafast MRM transition speeds, up to 555 MRMs per second, are achieved by Shimadzu’s UFsweeper™ collision cell technology, proprietary high-precision quadrupole machining capabilities, and unique high voltage power supply technology. In addition, the LCMS-8040 features the world’s fastest* polarity switching at 15 msec. With this high-speed performance, the LCMS-8040 can dramatically improve analytical throughput.

*Per survey result as of May 2012

Ultra-High Reliability

MRM optimization in Shimadzu’s LCMS systems is based on a rapid series of automated flow injection analyses, requiring only minutes to perform. Multiple compounds can be optimized in an unattended sequence, freeing the analyst from tedious work. MRM parameters optimized for the LCMS-8030 can be transferred to the LCMS-8040, making it possible to transfer methods between systems. The LCMS-8040 offers the same ease of maintenance benefits as the LCMS-8030, and all consumables, such as desolvation lines (DL) and ESI capillaries, are interchangeable as well.
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LCMS-IT-TOF

The LCMS-IT-TOF is a new type of mass spectrometer that combines QIT (ion trap) and TOF (time-of-flight) technologies. When combining QIT and TOF, the greatest technological challenges were the efficient introduction of ions into the QIT and the simultaneous ejection of trapped ions to the TOF. Here, we introduce the newly developed technologies used to solve these problems.

LCMS-IT-TOF

The source design, desolvation capillary and reduced collision ion focusing Q-Array were adapted from Shimadzu‘s successful single-quad HPLC mass spectrometer. The Octapole Lens can introduce eluted ions from the LC to the ion trap by CII (Compressed Ion Injection) method efficiently. The ion trap QIT gives an efficient MSn capability with our new cleaning technology. In addition, BIE (Ballistic Ion Extraction) technology, provides high resolution / accuracy for all MS, MSn… modes. The temperature control of the flight tube gives a mass stability with stabilizing the length of the ion flight and ion acceleration voltage which affects the mass stability. Dual-Stage Reflectron gives a excellent resolution with optimizing energy absorption and time convergence.
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